Part:BBa_K1846007:Design

Bacteriophage lambda tail fibre assembly (tfa) under TetR regulation

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We have combined the P(Cat)+TetR circuit BBa_K1846003 and the tfa circuit BBa_K1846001 into a composite part using a 3A assembly. The cloning procedure was confirmed by restriction with EcoRI and SpeI restriction enzymes and followed by agarose gel electrophoresis (Figure 1). The construct was also confirmed by sequencing. This composite part has been registered as BBa_K1846007.

Source

2015 iGEM distribution kit and DNA synthesis